Mammalian Transient Protein Expression
Fusion Antibodies delivers expert mammalian transient gene expression (TGE) for rapid, scalable protein generation. Our platform supports assay development, antibody screening, structural biology, and preclinical studies—with speed, precision, and reproducibility.
Mammalian Transient Protein Expression
Fusion Antibodies delivers expert mammalian transient gene expression (TGE) for rapid, scalable protein generation. Our platform supports assay development, antibody screening, structural biology, and preclinical studies—with speed, precision, and reproducibility.
Why Choose Fusion?
Proven Expertise Across Antibody Formats
We specialize in expressing and purifying complex antibody formats, including bispecifics with mispaired species, Fc-modified constructs, animal-derived antibodies, and miniature formats with atypical binding profiles.
Our team tailors purification strategies to each molecule, ensuring accurate recovery of correctly assembled products, even when standard affinity columns fall short.
Optimized Cell Culture
Our proprietary system combines the high-efficiency pETE vector with tailored reagents, media, and culture conditions. The result: high-yield expression with proper folding and post-translational modifications—ideal for dense mammalian systems.
Pre-expression Design Support
Not all proteins express easily. We offer in silico sequence analysis and construct optimization (e.g. fusion tags, codon usage) to improve expression and purification success—reducing risk and saving time.
Custom Purification Workflows
From standard antibodies to complex formats like bispecifics, we tailor purification using:
- Protein A/G affinity chromatography
- Size exclusion, ion exchange, and hydrophobic interaction
- Antigen-specific affinity purification
We isolate correctly assembled products and remove unwanted isoforms or aggregates.
Standard QC and Optional Analytics
All standard deliveries include:
- UV-Vis quantification
- SDS-PAGE
- Analytical SEC
- Endotoxin levels guaranteed <2 EU/mg.
- Full batch reports provided.
Enhance your project with:
- DSF for thermal stability
- Binding assays (ELISA, BLI)
- Endotoxin testing
Service Snapshot
| Feature | Details |
|---|---|
| Proteins expressed | 5,000+ (cumulative) |
| Turnaround | 4–8 weeks |
| Expression scale | 25 mL to 30 L |
| Cell lines | HEK293 or CHO |
| Endotoxin levels | <2 EU/mg |
| Custom purification & analytics | Available on request |
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