Mammalian Transient Protein Expression

Why Choose Fusion?

Proven Expertise Across Antibody Formats

We specialize in expressing and purifying complex antibody formats, including bispecifics with mispaired species, Fc-modified constructs, animal-derived antibodies, and miniature formats with atypical binding profiles.

Our team tailors purification strategies to each molecule, ensuring accurate recovery of correctly assembled products, even when standard affinity columns fall short.

Optimized Cell Culture

Our proprietary system combines the high-efficiency pETE vector with tailored reagents, media, and culture conditions. The result: high-yield expression with proper folding and post-translational modifications—ideal for dense mammalian systems.

Pre-expression Design Support

Not all proteins express easily. We offer in silico sequence analysis and construct optimization (e.g. fusion tags, codon usage) to improve expression and purification success—reducing risk and saving time.

Custom Purification Workflows

From standard antibodies to complex formats like bispecifics, we tailor purification using:

• Protein A/G affinity chromatography
• Size exclusion, ion exchange, and hydrophobic interaction
• Antigen-specific affinity purification

We isolate correctly assembled products and remove unwanted isoforms or aggregates.

Standard QC and Optional Analytics

All standard deliveries include:

• UV-Vis quantification
• SDS-PAGE
• Analytical SEC
• Endotoxin levels guaranteed <2 EU/mg.
• Full batch reports provided.

Enhance your project with:

• DSF for thermal stability
• Binding assays (ELISA, BLI)
• Endotoxin testing

Service Snapshot